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Acta Parasitologica, Vol.43, No. 3, 1998, 167-170
Hadas Edward (1), Rabel Bandule (2), Stankiewicz Miroslaw (2)* - The isolation and identification of prostaglandins of Trichostrongylus colubriformis and Haemonchus contortus.
(1) Department of Biology and Medical Parasitology, K. Marcinkowski University of Medical Sciences, Fredry 10, 61-701 Poznan, Poland; (2) AgResearch, Wallaceville Animal Research Centre, P.O. Box 40063, Upper Hutt, New Zealand
* present address Animal and Food Sciences Division, Lincoln University, P.O.Box 84, Canterbury, New Zealand, E-mail: stankiem@lincoln.ac.nz
ABSTRACT

Prostaglandins (PGs) have been implicated as precursors of biological processes, many of which are functional in parasite-host interactions. The aim of the study was the isolation and examination of PGs in extracts from adults, larvae and excretory/secretory products (E/S) of Trichostrongylus colubriformis and Haemonchus contortus. Thin-layer chromatographic (TLC) and high performance liquid chromatographic (HPLC) methods established the presence of PGs in the extracts that were identified as PGA2, PGB2, PGD2, PGE2, PGF1alpha, PGF2alpha, PGI2 and non-identified forms of PGs, precursors or metabolites of PGs. The qualification/quantitative profiles for the PGs were very similar for adult, L3 and E/S products of T. colubriformis and H. contortus.

KEY WORDS: prostaglandins, Trichostrongylus colubriformis, Haemonchus contortus
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