Strongyloidiasis frequently causes chronic asymptomatic disease in the gastrointestinal tract. Saliva samples have been recommended for antibody detection due to the ease of acquisition, non-invasive collection, and low risk of infection to the laboratory technicians. The present study was elaborated to examine the potential effectiveness of saliva samples for the detection of strongyloidiasis. A total of 118 paired human serum and saliva samples were analyzed to detect IgA and IgG anti- Strongyloides by the IFAT and ELISA using Strongyloides ratti antigen. By IFAT 54.2% of serum samples and 72.9% of salivary samples were specific IgA positive, while IgG positivity was 70.9% in serum and 33.3% in saliva samples. In ELISA, the positivity for serum IgA was 66.7% and 45.8% for salivary IgA, whereas specific IgG positivity was 77.1% in serum and 29.2% in saliva. It was concluded that IFAT, to detect specific IgA in saliva, and ELISA, to detect specific serum IgG, may be the more useful tests. Saliva can thus be used as an alternative fluid, in the application of IFAT, for strongyloidiasis identification.