Acta Parasitologica, Vol. 50, No. 3, 2005, 249-254 Elzbieta Hiszczynska-Sawicka(1), Jozef Kur(1), Halina Pietkiewicz(2), Lucyna Holec(1), Artur Gasior(1) and Przemyslaw Myjak(2)* - Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis
(1)Department of Microbiology, Technical University of Gdansk, 11/12 Narutowicza Street, 80-952 Gdansk; (2)Department of Tropical Parasitology, Inter-Faculty Institute of Maritime and Tropical Medicine, Medical University of Gdansk, 9b Powstania Styczniowego Street, 81-519 Gdynia; Poland
*Corresponding author: pemyjak@amg.gda.pl
ABSTRACT
The description of very efficient system for production and purification of Toxoplasma gondii recombinant antigens, GRA6, p35 and SAG2 is given in this study. The usefulness of these antigens for diagnostic of human infections was tested in an ELISA using 99 sera obtained during routine diagnostics. The sera for testing were selected from either acute or chronically infected patients. Both r-GRA6 and r-p35 antigens detected antibodies more frequently (p<0.01) from acute (93.9 and 87.9%) rather than chronic (60.6 and 53.0%) infections. The r-SAG2 gave a similar sensitivity in both groups of patients (93.9 and 95.5%).
KEY WORDS: ELISA, fusion protein, serological detection, Toxoplasma gondii recombinant antigens, GRA6, p35, SAG2